Welcome to Tissue Culture Microscopy
Welcome to the exciting biological science of tissue culture microscopy. The intent of this website is to provide a wide selection of information relating to cell culture microscopy. A tissue culture microscope consists of a compound light microscope with high power objectives facing upward. This is called an inverted tissue culture microscope. The inverted objective design is required because the microscope is used to view the bottom of a petri dish or flask since the culture is growing on the bottom of the dish. Normally, the magnifications go up to about 400x maximum due to the issue of objective working distance.
The higher the power of objective, the closer the objective needs to be to the specimen. Viewing a living cell culture specimen in the bottom of a petri dish requires and objective that has a long enough working distance that it can see past the thickness of the glass petri dish. A 40x objective is about the maximum magnification objective that still has a long enough working distance. All the objectives would be specially designed to be long working distance. Living cell tissue cultures are often opaque to view, and may need the inverted tissue culture microscope to have a phase contrast condenser. This introduces the concept of phase contrast microscopy. This method of microscopy is common when viewing living specimens that have little contrast in their unstained state. The phase contrast condenser and phase objective are used in conjunction with a phase annulus ring in the light condenser to give a phase contrast viewng system.
Tisssue culture microscopy is a useful scientific application for research and study of living cell cultures for microbiology classes, immunology research, biomedical research, and pharmaceutical drug testing. To expand this subject more, a tissue culture microscope may be fitted with an epi fluorescence microscope illumination set, dichroic fluorescent filter cube assembly, and fluorescent microscope objectives. An inverted tissue culture microscope fitted with these accessories becomes an inverted epi fluorescence microscope. Please contact us if you have a tissue culture microscopy application and are needing suitable equipment. We would be happy to assist you with finding low cost, economical tissue culture microscope that fits your needs.
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